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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 681891, 12 pages
http://dx.doi.org/10.1155/2013/681891
Research Article

The Antibacterial Effect of Ethanol Extract of Polish Propolis on Mutans Streptococci and Lactobacilli Isolated from Saliva

1Department of Conservative Dentistry with Endodontics, Division of Medicine and Dentistry, Medical University of Silesia, Pl. Akademicki 17, 41-902 Bytom, Poland
2Department of Pathology, School of Pharmacy and Laboratory Medicine, Medical University of Silesia, ul. Ostrogorska 30, 41-200 Sosnowiec, Poland
3Department and Institute of Microbiology and Virology, School of Pharmacy and Laboratory Medicine, Medical University of Silesia, ul. Jagiellońska 4, 41-200 Sosnowiec, Poland
4Department of Oral Surgery, Division of Medicine and Dentistry, Medical University of Silesia, Pl. Akademicki 17, 41-902 Bytom, Poland

Received 10 January 2013; Revised 5 February 2013; Accepted 25 February 2013

Academic Editor: Zenon Czuba

Copyright © 2013 Arkadiusz Dziedzic et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Dental caries occurrence is caused by the colonization of oral microorganisms and accumulation of extracellular polysaccharides synthesized by Streptococcus mutans with the synergistic influence of Lactobacillus spp. bacteria. The aim of this study was to determine ex vivo the antibacterial properties of ethanol extract of propolis (EEP), collected in Poland, against the main cariogenic bacteria: salivary mutans streptococci and lactobacilli. The isolation of mutans streptococci group bacteria (MS) and Lactobacillus spp. (LB) from stimulated saliva was performed by in-office CRT bacteria dip slide test. The broth diffusion method and AlamarBlue assay were used to evaluate the antimicrobial activity of EEP, with the estimation of its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The biochemical composition of propolis components was assessed. The mean MIC and MBC values of EEP, in concentrations ranging from 25 mg/mL to 0.025 mg/mL, for the MS and LB were found to be 1.10 mg/mL versus 0.7 mg/mL and 9.01 mg/mL versus 5.91 mg/mL, respectively. The exposure to an extract of Polish propolis affected mutans streptococci and Lactobacillus spp. viability, exhibiting an antibacterial efficacy on mutans streptococci group bacteria and lactobacilli saliva residents, while lactobacilli were more susceptible to EEP. Antibacterial measures containing propolis could be the local agents acting against cariogenic bacteria.