Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 1

Research Article

Hepatitis B Virus-Encoded X Protein Downregulates EGFR Expression via Inducing MicroRNA-7 in Hepatocellular Carcinoma Cells

Figure 1

The protein expression of EGFR was attenuated in response to HBx expression in HCC cells. (a) The protein expressions of EGFR, HBx, and Tubulin in HCC cells were analyzed by Western blot. Tubulin acts as an internal control. (b) Hep3B cells were transiently transfected with myc-HBx expression vector for 48 hrs. EGFR protein expression was examined by Western blot. (c) Hep3Bx cells were transiently transfected with si-control or si-HBx for 3 days. The gene silencing for HBx mRNA was determined by RT-qPCR. Under the condition, EGFR protein expression was also analyzed by Western blot. Statistical analysis was performed by Student’s -test. * as compared to the control group.

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