Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 3

Research Article

Hepatitis B Virus-Encoded X Protein Downregulates EGFR Expression via Inducing MicroRNA-7 in Hepatocellular Carcinoma Cells

Figure 3

HBx upregulated miR-7 expression to reduce EGFR protein level in HCC cells. (a) The miR-7 expression in HCC cells was examined by RT-qPCR. The miR-7 expression was normalized to small RNA U47 level. Statistical analysis was performed by Student’s -test. ** ; *** as compared to individual parental cells. (b) Hep3Bx cells were transiently transfected with si-control or si-HBx for 3 days. The miR-7 expression was analyzed by RT-qPCR. The miR-7 expression was normalized to small RNA U47 expression. Statistical analysis was performed by Student’s -test. ** as compared to control group. (c) Hep3B and Hep3Bx cells were transiently transfected with miR-7 mimic or miR-7 inhibitor, respectively. Four days later, the EGFR protein expression was analyzed by Western blot.
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