Hepatitis B Virus-Encoded X Protein Downregulates EGFR Expression via Inducing MicroRNA-7 in Hepatocellular Carcinoma Cells
Figure 4
The regulatory trait of HBx-miR-7-EGFR conferred HCC cells a slow growth behavior. (a) The growth curves of Hep3B, HepG2, and their derivatives were determined by MTT assay. Statistical analysis was performed by Student’s -test. *; ** as compared to individual parental cells. (b) Hep3Bx cells were transiently transfected with si-control and si-HBx for 4 days. These cells were then trypsinized for cell number counting. Statistical analysis was performed by Student’s -test. ** as compared to control group. (c) Hep3Bx cells were transiently transfected with or without miR-7 inhibitor for 1 day. Cells were reseeded at the same amount between groups and allowed for growth. The growth rate was determined by crystal violet staining. Total RNA was also collected for examination of miR-7 expression by RT-qPCR. (d) Hep3Bx cells were transiently transfected with or without myc-EGFR expression vector for 1 day. Similar procedures as described in (c) were performed. The protein expression of myc-EGFR was examined by Western blot.
