The Inhibitory Effect of 3β-Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway
Figure 9
Effect of 3β-hydroxy-12-oleanen-27-oic acid (ATA) on MAPK signal pathway in HepG2 cells detected by Western blot. (a) HepG2 cells were treated with 10 μg/mL of ATA for 0, 12, and 24 h, respectively. Then the cells were harvested and the total protein was separated by SDS-PAGE. Total and phosphorylated expression levels of the MAPK family members (JNK, p38, ERK1/2, p-JNK, p-p38, and p-ERK1/2) were examined. (b) HepG2 cells were treated with 5 and 10 μg/mL of ATA for 24 h, respectively. (c) HepG2 cells were treated with the JNK inhibitor SP600125 (10 μM) for 1 h before treatment with 10 μg/mL of ATA for 24 h and p-JNK expression level was determined. Actin was used as an internal control. The figures shown were representative of three independent experiments.
