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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 686018, 15 pages
Research Article

Immunomodulation of Homeopathic Thymulin 5CH in a BCG-Induced Granuloma Model

1Research Center of Universidade Paulista, Rua Dr. Bacelar 1212, 5th Floor, 04026-002 São Paulo, SP, Brazil
2Laboratory of Veterinary Pathology, Universidade de Santo Amaro, São Paulo, SP, Brazil
3Instituto de Ensino, Pesquisa e Desenvolvimento Royal, São Roque, SP, Brazil
4Laboratory of Immunology, Universidade Federal de São Paulo, São Paulo, SP, Brazil

Received 9 October 2012; Revised 3 December 2012; Accepted 20 December 2012

Academic Editor: Ke Ren

Copyright © 2013 Leoni Villano Bonamin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present study analyzed the immune modulation mechanisms of thymulin 5CH in a granuloma experimental model. Male adult Balb/c mice were inoculated with BCG into the footpad to induce granuloma, which was quantitatively evaluated. The phenotypic characterization of phagocyte, T- and B-lymphocyte populations in the peritoneum, and local lymph node was done by flow cytometry. During all experimental periods, thymulin 5CH and vehicle (control) were given ad libitum to mice, diluted into the drinking water ( M). After 7 days from inoculation, thymulin-treated mice presented reduction in the number of epithelioid cytokeratine-positive cells () in the lesion, in relation to young phagocytes. After 21 days, the differentiation of B1 peritoneal stem cells into phagocytes reached the peak, being higher in thymulin-treated mice (). Simultaneously, the score of infected phagocytes in the lesion decreased (), and the number of B1-derived phagocytes, CD4+ and CD8+ T lymphocytes in the local lymph node increased in relation to control (). No difference was seen on the CD25+ Treg cells. The results show that thymulin 5CH treatment is able to improve the granuloma inflammatory process and the infection remission, by modulating local and systemic phagocyte differentiation.