Research Article

Ginsenoside RK3 Prevents Hypoxia-Reoxygenation Induced Apoptosis in H9c2 Cardiomyocytes via AKT and MAPK Pathway

Figure 3

Effects of H/R on apoptosis in H9c2 cardiomyocytes. H9c2 cardiomyocytes were exposed to 6 h of hypoxia followed by 24 h of reoxygenation. Then, the caspase-3 activity (units per microgram of protein) was assayed, as described in Section 2, at 0, 2, 4, 8, 12, and 24 h after reoxygenation (a). Hoechst 33342 and PI double staining were also used in the qualitative and quantitative analyses of the apoptotic cells (b and c). The results are represented as means ± SD from three independent experiments. * versus control group.
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