Bromelain Inhibits Allergic Sensitization and Murine Asthma via Modulation of Dendritic Cells

Figure 6

sBr treatment of DCs in vitro results in reduced expression of CD44. MLN DCs were selected using Pan DC Beads and cells (5 × 10⁵/well) were cultured overnight. (a) Representative histogram of CD44 expression on cells in media alone (MFI 2668), sBr 100 μg/mL treated cells MFI (1198), and negative control (MFI 244). (b) denotes MFI of CD44 and (c) % cell expression of CD44 on cells alone, sBr (1−100 μg/mL), and (E64 treated sBr 100 μg/mL). Gates were on live, CD3⁻CD11c⁺MHCII⁺ cells; MFI: mean fluorescence intensity. ^∗𝑃<0.01; ^∗∗𝑃<0.0001 as compared to control cells in media alone. Data represents triplicate wells of duplicate experiments.

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