Inhibition of NADPH Oxidase Mediates Protective Effect of Cardiotonic Pills against Rat Heart Ischemia/Reperfusion Injury

Figure 10

CP pretreatment inhibits NADPH oxidase activation induced by I/R. (a) Representative Western blot bands of gp91^phox, p67^phox, p47^phox and p40^phox on cell membrane. (b) Quantification of cell membrane expression of gp91^phox, p67^phox, p47^phox and p40^phox. All membrane protein intensities were normalized to GAPDH. (c) Representative Western blot bands of NADPH oxidase organizer subunits p67^phox and p47^phox and modulator p40^phox in cytoplasm. (d) Statistical results of cytoplasm expression of p67^phox, p47^phox and p40^phox. All band intensities were calculated based on the results from 3 independent experiments. All cytosolic protein intensities were normalized to 𝛽-tubulin. Results are presented as mean ± SEM (𝑛=3). ^∗𝑃<0.05 versus Sham group, ^#𝑃<0.05 versus I/R group.

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