Interaction of Veratrum nigrum with Panax ginseng against Obesity: A Sang-ban Relationship
Figure 3
Effects of the water extract of VN on adipogenesis and transcription factors in 3T3-L1 cells. An MTS assay (a), Oil Red O staining assay (b), real-time RT-PCR assay (c), and Western blot assay (d) were performed to measure the effects of VN in 3T3-L1 cells. 3T3-L1 preadipocytes were induced to differentiate with 10% FBS/DMEM medium containing insulin, DEX, IBMX, and 0, 0.01, 0.1, or 1 mg/mL of VN extract for 2 days, and then the culture media were replaced with 10% FBS/DMEM medium containing insulin for the following 2 days. The control was treated with DW instead of extracts. EGCG was used as a positive control. Assays were performed in duplicates for each concentration, and experiments were repeated at least three times. Data represented are the relative expression. All values are mean ± SD. #, versus undifferentiated control cells; * and **, versus differentiated control cells; DW, distilled water; VN, Veratrum nigrum; EGCG, epigallocatechin gallate.
