Interaction of Veratrum nigrum with Panax ginseng against Obesity: A Sang-ban Relationship
Figure 4
Comparison of the effects of the mixture and the combination extract of PG and VN on differentiation of 3T3-L1 cells. An MTS assay (a) was performed to measure the effects of PG-VN combination in 3T3-L1 cells. A real-time RT-PCR assay was performed to measure the difference between the effects of the combination and the mixture on PPAR and C/EBP mRNA expressions in 3T3-L1 cells (b). 3T3-L1 preadipocytes were induced to differentiate with 10% FBS/DMEM medium containing insulin, DEX, IBMX, and 0, 0.01, 0.1, or 1 mg/mL of PG-VN mixture or PG-VN combination for 2 days, and then the culture media were replaced with 10% FBS/DMEM medium containing insulin for the following 2 days. The control was treated with DW instead of extracts. EGCG was used as a positive control. Assays were performed in duplicates for each concentration, and experiments were repeated at least three times. Data represented are the relative expression. All values are mean ± SD. #, versus undifferentiated control cells; * and **, versus differentiated control cells; DW, distilled water; PG, Panax ginseng; VN, Veratrum nigrum; 1 : 1, PG-VN combination.
