Research Article

Saikosaponin-d Enhances the Anticancer Potency of TNF- via Overcoming Its Undesirable Response of Activating NF-Kappa B Signalling in Cancer Cells

Figure 3

Down-regulatory effect of Ssd on the TNF-α-induced NF-κB-dependent genes expression. (a) Ssd downregulates TNF-α-induced NF-κB dependent cell proliferative genes expression. (b) Ssd downregulates TNF-α-induced NF-κB-dependent invasive genes expression. (c) Ssd downregulates TNF-α-induced NF-κB-dependent angiogenic genes expression. HepG2 and HeLa cells were pretreated with 20 ng/mL TNF-α for 60 min and then subjected to 10 μM of Ssd treatment as indicated in time intervals, and the whole cell extracts were prepared and analyzed by Western blotting. Gel images are representative of three independent experiments.
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