Sargassum fulvellum Protects HaCaT Cells and BALB/c Mice from UVB-Induced Proinflammatory Responses
Figure 3
Inhibition of UVB-induced iNOS expression and subsequent NO production by SFE-EtOAc in HaCaT cells. HaCaT cells were pretreated with vehicle or SFE-EtOAc (30 μg/mL and 100 μg/mL) for 9 h and then exposed to UVB (60 mJ/cm2). Thereafter, media were replaced with fresh media containing SFE-EtOAc or vehicle, and cells were additionally incubated for 24 h. (a) Western blot analysis was performed using anti-iNOS or antiactin primary antibody. Representative blots from three independent experiments are shown. The actin levels were monitored for the confirmation of equal amount of protein loading. (b) The NO levels released into the medium were measured by Griess assay as described in Section 2. The relative levels of the NO production were represented as fold induction in comparison with the vehicle-treated control cells ( μM). Asterisks indicate a significant difference compared with UVB-irradiated group (**).
