Clitocybe nuda Activates Dendritic Cells and Acts as a DNA Vaccine Adjuvant
WE-CN upregulates the expression of immunomodulatory cell surface markers on BMDCs. Immature BMDCs were stimulated with 100 ng/mL LPS or 100 μg/mL WE-CN for 24 hr. The control group was treated with PBS alone. After incubation, the expression of the surface markers CD40, CD80, CD86, MHC class I, and MHC class II was analyzed by flow cytometry with fluorescently labeled Abs. All data were gated on CD11c+ cells. The gray-filled area represents staining with an isotype-matched control Ab. (a) The histogram shows data from one representative experiment of each group. (b) The bar graphs represent the mean ± SD from three independent experiments.