[6]-Gingerol Prevents Disassembly of Cell Junctions and Activities of MMPs in Invasive Human Pancreas Cancer Cells through ERK/NF-κB/Snail Signal Transduction Pathway
Figure 3
Effect of [6]-gingerol on TER value and cell invasion in PANC-1 cells. (a) Cells were plated onto 12-well polyester membrane Transwells (1 cm2 surface area, 0.4-mm pore size; Costar) and grown in media. The compound was added to both the apical and basolateral compartments in triplicate. TER values were measured using an epithelial volt-ohm meter. (b) Cells were grown on the apical side of a Matrigel-coated filter chamber in the presence of the compound in serum-free media. Medium including 20% FBA as a chemoattractant was placed in the basolateral chamber. After 3 days, cells were fixed and stained and then counted. Data represent the mean ± SD from three independent experiments. Statistical analyses (one-way ANOVA and Student's -test) were performed using GraphPad Prism 5. * versus the untreated control.
