[6]-Gingerol Prevents Disassembly of Cell Junctions and Activities of MMPs in Invasive Human Pancreas Cancer Cells through ERK/NF-κB/Snail Signal Transduction Pathway
Figure 6
Effect of [6]-gingerol on TJ-related gene expression in PANC-1 cells. (a) Cells were treated with [6]-gingerol for 24 h. Total RNAs were extracted and reverse transcribed. cDNAs were subjected to PCR, and products were observed by 1.5% agarose gel electrophoresis and visualized by staining with EtBr. GAPDH was used as an internal control. (b) Cells cultured under the same conditions were lysed, and equal amounts of cell protein were resolved by SDS-PAGE and transferred to a nitrocellulose membrane. Western blotting was then performed with the proper antibodies and an enhanced ECL detection system. Actin was used as an internal control. Band intensities in the immunoblots were quantified by densitometry using L Process and MultiGauge software. Band intensities were normalized relative to the internal control and background, respectively. Data represent the mean ± SD from three independent experiments. Statistical analysis (Student's -test) was performed using GraphPad Prism 5. * versus the untreated control.
