Efficacy and Safety of Gwakhyangjeonggi-San Retention Enema in Normal Rats and Spontaneously Hypertensive Rats

Table 1

The setting of drug retention enema.

	The kind, quality of the material and diameter of catheter	The insertion length of catheter	Sample volume	Retention time	Instillation speed
Setting	8 Fr suction catheter	8 cm	6 mL/300 g	10 min	4 mL/min
Procedure	(i) S.D rat (280–320 g)
	(ii) Measuring BW
	(iii) Anesthetizing by 2% isoflurane
	(iv) Ventral position, angle (45°)
	(v) Connecting syringe which is filled with sample or 0.9% saline to catheter and inserting it into the anal of S.D rat (8 fr suction catheter, 8 cm)
	(vi) Plastering baseline in catheter
	(vii) Instillation sample or 0.9% saline (6 mL/300, 4 mL/min)
	(viii) Retaining sample or 0.9% saline during 10 min
	(ix) Recovering S.D rat during 10 min (defecation)
	(x) Measuring BW