Recombinant Protein Production of Earthworm Lumbrokinase for Potential Antithrombotic Application

Table 1

Lumbrokinase gene clones, expression, and characterization.

Earthworm	Lumbrokinase	GenBank no.	Expression host(s)	Characterization references
L. bimastus	PI239	AF433650	P. pastoris; E. coli	Ge et al., 2005 [22]; Xu et al., 2010 [23]
	PM246	AY187629	P. pastoris	Hu et al., 2005 [21]
	PV242	AF109648	E. coli	Xu et al., 2002 [17]
L. rubellus	F-III-1	AB045720
	F-III-2	AB045719	P. pastoris; E. coli	Sugimoto and Nakajima 2001 [24]; Li et al., 2008 [25]
	1T4	U25643
	lk-6 (F6)	AF304199	E. coli	Cho et al., 2004 [26]
	EFE3-1	U25648 (AY327442)*	Goat mammary glands; Sunflower plant	Hu et al., 2004 [20]; Guan et al., 2013 [27]
E. fetida	CST1	AY840996	E. coli	Li et al., 2012 [15]
	CST2-2	AY684712
	CST2-1	AY684711
	TFe	EU167737
	Tfd	EU167736
	Tfc	EU167735
	Tf2	EU167734
	F238	DQ202401	P. pastoris	Zhao et al., 2006 [28]
	efp-1	DQ418454
	efp-0	DQ836917
	a1	AF393512
	EFE-3	AY438622	E. coli; P. pastoris	Dong et al., 2004 [29]; Yuan et al., 2006 [30]
		AF432224
		AY438624
		AY438623
		AY438625

∗(AY327442) was codon optimized from U25648.