Effect of Gambisan on the Inhibition of Adipogenesis in 3T3-L1 Adipocytes

Table 1

Gene-specific forward and reverse primer sequences used for RT-PCR analysis.

Target gene names	Direction	Primer sequence	Annealing time (cycles)
HSL	Forward	5′-GAGGGACACACACACACCTG-3′	55°C (30)
	Reverse	5′-CCCTTTCGCAGCAACTTTAG-3′
LPL	Forward	5′-AGTAGACTGGTTGTATCGGG-3′	55°C (30)
	Reverse	5′-AGCGTCATCAGGAGAAAGG-3′
β-actin	Forward	5′-AGCCATGTACGTAGCCATCC-3′	55°C (32)
	Reverse	5′-TCCCTCTCAGCTGTGGTGGTGAA-3′

HSL: hormone-sensitive lipase, LPL: lipoprotein lipase, RT-PCR: reverse transcription-polymerase chain reaction.