Effect of Angelica sinensis Polysaccharides on Osteoarthritis In Vivo and In Vitro: A Possible Mechanism to Promote Proteoglycans Synthesis

Figure 4

Effect of Angelica sinensis polysaccharides (APS-3c) on the expression of chondrocytes specific genes involved in proteoglycan-degrading enzymes of rat interleukin-1-beta- (IL-1β-) stimulated chondrocytes. (a) RT-PCR of aggrecanase-1, aggrecanase-2, MMP-3 and GAPDH (from top to bottom) mRNA in response to various concentrations of APS-3c. Integrated density values for polymerase products were normalized to the values for GAPDH. ((b), (c), and (d)). The resulting data were expressed and illustrated as a ratio of the normal control. Values represent Mean ± SEM of four different simples. ^∗∗𝑃<0.01versus normal control. ^##𝑃<0.01versus IL-1β-stimulated control.

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