Effect of Angelica sinensis Polysaccharides on Osteoarthritis In Vivo and In Vitro: A Possible Mechanism to Promote Proteoglycans Synthesis
Figure 5
Effect of Angelica sinensis polysaccharides (APS-3c) on the protein expression of O-xylosyltransferase I (XylT-I), β1,3-glucuronosyltransferase I (GlcAT-I) and β-actin in rat interleukin-1-beta-(IL-1β-) stimulated chondrocytes. Expressions of proteins determined by western blotting. (a) Western blotting of O-xylosyltransferase I (XylT-I), β1,3-glucuronosyltransferase I (GlcAT-I) and β-actin (from top to bottom) protein in response to various concentrations of APS-3c. ((b) and (c)) Relative level of XylT-I and GlcAT-I normalized to β-actin and compared with normal control, quantitatively analyzed by Kodak Digital Science 1D software (Eastman Kodak, Rochester, NY, USA) and expressed as mean optical density. Values represent mean ± standard error of the mean of three different simples. versus normal control. versus IL-1β-stimulated control.
