Research Article

Antioxidant Effects of Quercetin and Naringenin Are Associated with Impaired Neutrophil Microbicidal Activity

Figure 4

Effects of naringenin and quercetin on ROS production by neutrophils. (a) Neutrophils were preincubated in the presence or absence of naringenin and quercetin (10 and 100 μM) and stimulated with PMA (400 nM), and intracellular ROS production was determined by flow cytometry using DHR as a probe. (b) Representative histograms are shown in logarithmic scale. Neutrophils were preincubated in the presence of naringenin 10 μM (A) and 100 μM (B) and activated with PMA. Neutrophils were preincubated in the presence of quercetin 10 μM (C) and 100 μM (D) and activated with PMA. Neutrophils (negative control) (E); neutrophils activated with PMA (positive control) (F). The percentage of inhibition of ROS production by naringenin and quercetin was calculated and compared with the positive group. The data are expressed as the mean ± SEM of four experiments. , 10 μM naringenin versus 10 μM quercetin and 100 μM naringenin versus 100 μM quercetin.
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