BNC Protects H9c2 Cardiomyoblasts from H₂O₂-Induced Oxidative Injury through ERK1/2 Signaling Pathway

Figure 7

BNC protected H9c2 cells from H₂O₂-induced apoptosis, as detected by Annexin V-FITC and PI staining with FCM analysis. (a) H9c2 cells without BNC or H₂O₂ (control); (b) H9c2 cells exposed to 100 μM H₂O₂ (model); ((c)–(h)) H9c2 cells pretreated with 0, 7.81, 15.63, 31.25, 62.50 μg/mL BNC, or 10 μM TMZ followed by the treatment of 100 μM H₂O₂. Values are expressed as mean ± SD from three independent experiments. **𝑃<0.01 versus control; *𝑃<0.05 versus control; ^##𝑃<0.01 versus model; ^#𝑃<0.05 versus model.