Inclusion Complex of Zerumbone with Hydroxypropyl-β-Cyclodextrin Induces Apoptosis in Liver Hepatocellular HepG2 Cells via Caspase 8/BID Cleavage Switch and Modulating Bcl2/Bax Ratio

Figure 11

Western blot analysis on effect of ZER-HPβCD inclusion complex on the levels of apoptosis regulatory proteins in HepG2 cells. Bax was upregulated while Bcl-2 in contrast downregulated in a time-dependent manner. Hsp-70 was also found to be depleted as time increases. The blot densities are expressed as folds of control. Detection of proteins was done by specific antibodies with β-actin as a loading control. Data are mean ± SD (𝑛=3). ^∗𝑃<0.05 versus control.

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