Inclusion Complex of Zerumbone with Hydroxypropyl-β-Cyclodextrin Induces Apoptosis in Liver Hepatocellular HepG2 Cells via Caspase 8/BID Cleavage Switch and Modulating Bcl2/Bax Ratio
Figure 4
Confocal micrographs of acridine orange and propidium iodide double-stained HepG2 cells treated with 11.43 μg/mL ZER-HPβCD inclusion complex. (400x magnification) (a) Untreated cells showed normal structure without prominent apoptosis; (b) early apoptosis features were seen after 24 h representing intercalated acridine orange (bright green) amongst the fragmented DNA; (c) more cells showing membrane blebbing after 48 h; (d) presence of reddish-orange colour at 72 h. VC: viable cells; MB: cell membrane blebbing; CC: chromatin condensation; LA: late apoptosis.