Subtoxic Levels of Apigenin Inhibit Expression and Secretion of VEGF by Uveal Melanoma Cells via Suppression of ERK1/2 and PI3K/Akt Pathways
Figure 5
Dose effects of apigenin on phosphorylated Akt, ERK, JNK, and p38 MAPK levels in cultured uveal melanoma cells. Cells (SP6.5) were plated into 24-well plates. After 24 h incubation, apigenin at 0, 1, 2, and 5 μM was added to the medium. Cells were collected 60 min later. The amount of phosphorylated Akt (p-Akt), ERK1/2 (p-ERK), JNK1/2 (p-JNK), and p38 MAPK (p-p38) in cell lysates was measured using the relevant ELISA kits and was expressed as the percentages of the controls (cells cultured without apigenin). Apigenin significantly decreased p-Akt (a) and p-ERK levels (b) and increased p-JNK (c) and p-p38 (d) levels in cell lysates in a dose-dependent manner. Data are mean ± SD (). , versus control.
