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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 835854, 4 pages
http://dx.doi.org/10.1155/2013/835854
Research Article

Total Phenolics and Total Flavonoids Contents and Hypnotic Effect in Mice of Ziziphus mauritiana Lam. Seed Extract

1Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudthaya Road, Ratchathevi, Bangkok 10400, Thailand
2Department of Physiology, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudthaya Road, Ratchathevi, Bangkok 10400, Thailand

Received 1 March 2013; Accepted 22 May 2013

Academic Editor: Molvibha Vongsakul

Copyright © 2013 Aye Moh Moh San et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The seeds of Ziziphus mauritiana Lam. have been traditionally used for treatment of various complications including insomnia and anxiety. They are popularly used as sedative and hypnotic drugs in China, Korea, Myanmar, Vietnam, and other Asian countries. However, no scientific proof on hypnotic activity of Z. mauritiana seeds (ZMS) was reported. In this study, the hypnotic activity of 50% ethanolic extract from ZMS was observed on the loss of righting reflex in mice using pentobarbital-induced sleep mice method. The contents of total phenolics and total flavonoids in the extract were also determined. The results showed that the 50% ethanolic extract from ZMS contained total phenolics  mg gallic acid equivalent (GAE)/g extract and total flavonoids  mg quercetin equivalent (QE)/g extract. Oral administration of the extract at the dose of 200 mg/kg significantly increased the sleeping time in mice intraperitoneally administered with sodium pentobarbital (50 mg/kg body weight). These results supported the traditional use of ZMS for the treatment of insomnia. The seeds of Z. mauritiana should be further developed as an alternative sedative and/or hypnotic product.