Biochanin A Promotes Osteogenic but Inhibits Adipogenic Differentiation: Evidence with Primary Adipose-Derived Stem Cells
Figure 2
Biochanin A inhibited the expression of adipogenesis-related factors in ADSCs. ADSCs were treated for 12 days with a basic medium (negative control) or an adipogenic medium (MDI) in the presence of 0.1–1 μM biochanin A. After 12 days of incubation, the expression of PPARγ, LPL, leptin, and OPN mRNA was measured by RT-PCR. GAPDH expression was used for normalization. All results are expressed as the mean SD of three independent experiments. * compared with the control.
