Biochanin A Promotes Osteogenic but Inhibits Adipogenic Differentiation: Evidence with Primary Adipose-Derived Stem Cells
Figure 5
Biochanin A enhanced the expression levels of genes and proteins that regulate osteogenic differentiation in ADSCs. ADSCs were treated for 12 days with a basic medium (negative control) or an osteogenic medium (OM) in the presence of 0.1–1 μM biochanin A. (a) After incubation, expression of ALP, OCN, and GAPDH mRNA was measured by RT-PCR. Expression of ALP and OCN genes was normalized to that of GAPDH. (b) After incubation, total proteins were isolated and analyzed for the expression of OPG, Runx2, Sirt1, and RhoA proteins by western blot analysis. β-actin was used as the internal control. All results are expressed as the mean ± SD of three independent experiments. * compared with the control.
