Protective Effect of Standardized Extract of Ginkgo biloba against Cisplatin-Induced Nephrotoxicity

Figure 6

EGb suppressed CDDP-induced IκB degradation, p65 phosphorylation, and iNOS expression in LLC-PK1 cells. (a) LLC-PK1 cells were preincubated with EGb (200, 100, and 50 μg/mL) for 24 h and then incubated with 50 μM CDDP at 37°C for 12 h. IκB, iNOS, phospho-p65, p65, caspase-3, caspase-9, and caspase-8 protein levels from LLC-PK1 cells extracts were analyzed by western blotting. (b) Protein expressions were semiquantified by densitometry analysis. (c) iNOS mRNA levels were analyzed with RT-PCR and then semiquantified. Data represent mean ± S.D. from three independent experiments. ^∗𝑃<0.05 versus saline; ^#𝑃<0.05 versus CDDP.