Anti-Inflammatory Effects and Mechanisms of Fatsia polycarpa Hayata and Its Constituents
Figure 6
Inhibition of LPS-induced activation of the IKK/NF-κB pathway and MAPKs by MCE or HOEO. Western blot was utilized to assay the expression of the indicated proteins in RAW 264.7 cells. Experiments were performed in duplicate. Cells were treated with 100 μg/mL of MCE (lanes 5 and 6) or 20 μM of HOEO (lanes 7 and 8) for 3 h, followed by stimulation with LPS (lanes 3–8) for 1 h or 2 h (c). (a) The analysis of phosphorylated IKK (P-IKK) and total α and β subunits of the IKK complex (IKK-α and IKK-β). (b) The analysis of phosphorylated IκB (P-IκB) and actin. (c) The analysis of p65, lamin B, and actin in the nuclear and cytosolic fractions of cells, respectively. (d), (e), and (f), the analysis of phosphorylated ERK (P-ERK) and total ERK (d), phosphorylated JNK (P-JNK) and total JNK (e), and phosphorylated p38 (P-p38) and total p38 (f), respectively. Band intensities relative to lane 1 in each blot were determined after normalization by the corresponding total protein, actin or lamin B as indicated in the respective histogram.
