Therapeutic Effect of the Tuber of Alisma orientale on Lipopolysaccharide-Induced Acute Lung Injury
Figure 6
Effect of EEAO posttreatment on Nrf2 activation and Nrf2 dependent genes in the lung. (a) Nuclear proteins were extracted from the lungs of mice treated with LPS, in combination with increasing amounts of EEAO. The amounts of nuclear proteins were quantitated and analyzed by western blotting of Nrf2. The membrane was stripped and reprobed with α-lamin A/C for ensuring an equal loading of nuclear proteins. (b) Total RNA was extracted from the harvested lungs, and expressions of NQO-1, HO-1, and GCLC genes were analyzed by Semiquantitative RT-PCR. The intensity of each PCR band was measured by densitometric analysis (ImageJ) and normalized to GAPDH intensity. * was less than 0.01, and ** was less than 0.05, compared with the LPS treated (post-ANOVA comparison with Tukey’s post hoc test).
