Bee Venom Mitigates Cisplatin-Induced Nephrotoxicity by Regulating CD4+CD25+Foxp3+ Regulatory T Cells in Mice
Figure 5
The inefficacy of bee venom in CD25-depleted mice. All mice were treated with anti-CD25 antibody (0.1 mg/mouse, i.p.) twice, before and after BV injection. Nephrotoxicity was induced by cisplatin (25 mg/kg i.p.). Blood was obtained at 24, 48, and 72 h after cisplatin injection (). Renal dysfunction was reflected by the levels of creatinine at 48 h, 72 h (a) and BUN at 48 h, 72 h (b). Kidney sections from CD25-depleted mice were stained with H&E at 72 h after cisplatin injection. PBS treatment alone (cont); PBS and cisplatin treatments (Cis); BV and cisplatin (Cis + BV) (400x, bar = 50 μm) (c). Tubular injury damage was scored from 0–4 (0, none; 1, <10%; 2, 10–25%; 3, 25–75%; 4, >75%) (d). The values shown indicate the mean ± SEM. NS, versus Cis.
