Figure 5: Effect of PC-SPESII on MDA-MB-231 cell invasion. (a) Transwell chamber was used for the invasion assay and images were taken at 200x magnification. The filter membranes were coated with Matrigel. MDA-MB-231 cells were treated with 0 (B), 0.05 (C), 0.25 (D), 0.5 (E), or 1 (F) μL/mL of PC-SPESII for 24 hours. No cells were seeded in (A). (b) Stand and error bars represent three independent experiments and each experiment was performed in triplicate (* and ** compared to untreated control).