Ursolic-Acid-Enriched Herba Cynomorii Extract Protects against Oxidant Injury in H9c2 Cells and Rat Myocardium by Increasing Mitochondrial ATP Generation Capacity and Enhancing Cellular Glutathione Redox Cycling, Possibly through Mitochondrial Uncoupling
Figure 9
Effect of HCY2/UA pretreatment on (a) mitochondrial GSH/GSSG and (b) GR activity in control perfused and ischemic-reperfused hearts. The ratio of mitochondrial GSH/GSSG and the activity of GR were measured as described in Section 2. The values of mitochondrial GSH/GSSG of non-I/R control were 5.0 ± 0.21 (male HCY2), 4.4 ± 0.58 (male UA), 7.0 ± 0.99 (female HCY2), and 6.0 ± 0.38 (female UA). The values of mitochondrial GR activity of non-I/R control were 2.64 ± 0.11 (male HCY2), 2.38 ± 0.14 (male UA), 2.91 ± 0.06 (female HCY2), and 1.71 ± 0.03 (female UA) mU/mg protein. Each bar represents mean ± SEM, with . “*” significantly different from the non-I/R control; “#” significantly different from I/R-challenged control.
