Research Article

Epimedium Flavonoids Counteract the Side Effects of Glucocorticoids on Hypothalamic-Pituitary-Adrenal Axis

Figure 5

Migration of BrdU-positive cells toward the inner layer of adrenal gland. Rats were injected with BrdU; 14 days later, rats were killed and adrenal glands were excised, and the BrdU incorporation assay was carried out. ((a), (b), (c)) Sections of the adrenal gland from rats of the control group, corticosterone only-treated group, and combined corticosterone and EFs-treated group, respectively, were stained with anti-BrdU antibody and visualized with microscope (magnification ×400). (d) The percentage of BrdU-positive cells in zona glomerulosa or zona fasciculata against total BrdU-positive cells was calculated, respectively. Cells with brown nuclei are considered as BrdU-positive cells. Cap, G, and F denote the capsule, and zona glomerulosa, zona fasciculata, respectively. All sections were counterstained with hematoxylin.
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