Research Article

Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells

Figure 4

TRPV1 channel is involved in S-[6]-gingerol-induced expression of cIAP-2, XIAP, and Bcl-2 in HuH-7 cells. HuH-7 cells were pretreated with 40 μM capsazepine (CapZ) for 30 min before they were exposed to S-[6]-gingerol for 1 hour. After treatments, cells were harvested and total RNA extracted and normalised to 100 ng/μL. RT-qPCR was then used to measure the mRNA levels of cIAP-2, XIAP, and Bcl-2, normalised to the reference gene, beta 2 microglobulin (B2M). All results are expressed as mean ± SEM of 3 independent experiments. *   versus DMSO vehicle control. ***   versus DMSO vehicle control. # versus [6]-S-gingerol treatment. ###   versus S-[6]-gingerol treatment.
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