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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 960603, 11 pages
http://dx.doi.org/10.1155/2013/960603
Research Article

Modulation of the Expression of the Proinflammatory IL-8 Gene in Cystic Fibrosis Cells by Extracts Deriving from Olive Mill Waste Water

1Department of Life Science and Biotechnology, Laboratory of Medicinal and Molecular Biotechnology, University of Ferrara, Via Fossato di Mortara 74, 44121 Ferrara, Italy
2Health Products Laboratory, University of Ferrara, Via Fossato di Mortara 17/19, 44121 Ferrara, Italy

Received 7 February 2013; Revised 22 April 2013; Accepted 29 April 2013

Academic Editor: Chong-Zhi Wang

Copyright © 2013 Ilaria Lampronti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A persistent recruitment of neutrophils in the bronchi of cystic fibrosis (CF) patients contributes to aggravate the airway tissue damage, suggesting the importance of modulating the expression of chemokines, including IL-8 during the management of the CF patients. Polyphenols rich extracts derived from waste water from olive mill, obtained by a molecular imprinting approach, have been investigated in order to discover compounds able to reduce IL-8 expression in human bronchial epithelial cells (IB3-1 cells), derived from a CF patient with a ΔF508/W1282X mutant genotype and stimulated with TNF-alpha. Initially, electrophoretic mobility shift assays (EMSAs) were performed to determine whether the different active principles were able to inhibit the binding between transcription factor (TF) NF-kappaB and DNA consensus sequences. Among different representative active principles present in the extract, three compounds were selected, apigenin, oleuropein, and cyanidin chloride, which displayed remarkable activity in inhibiting NF-kappaB/DNA complexes. Utilizing TNF-alpha-treated IB3-1 cells as experimental model system, we demonstrated that apigenin and cyanidin chloride are able to modulate the expression of the NF-kappaB-regulated IL-8 gene, while oleuropein showed no effect in regulating the expression of the gene IL-8.