Figure 6: Effects of apigenin, oleuropein, and cyanidin chloride on IL-8 gene expression in TNF-α-treated cells. IB3-1 cells were exposed for 24 h to the indicated concentrations of compounds in the presence of TNF-α as described in methods. After this incubation time, RNAs and cellular supernatants were isolated. Quantitative RT-PCR was performed, and IL-8 mRNA content was quantified in respect to control uninduced IB3-1 cells ; -α-treated IB3-1 cells. ;   .