P90RSK and Nrf2 Activation via MEK1/2-ERK1/2 Pathways Mediated by Notoginsenoside R2 to Prevent 6-Hydroxydopamine-Induced Apoptotic Death in SH-SY5Y Cells
Figure 2
Protective effects of NGR2 on 6-OHDA-induced cell death in SH-SY5Y cells. Cell viability was measured by cell counting kit-8 test or LDH assay. (a) 6-OHDA could induce cell death in SH-SY5Y cells in concentration- and time-dependent manners. (b) NGR2 had no toxic effect on cell viability. Preincubation with different concentrations of NGR2 for different periods of time had protective effect on 6-OHDA-induced cell death (c) and LDH release (d) in SH-SY5Y cells. Coincubation with NGR2 had almost no protective effect on 6-OHDA-induced cell death (e) and LDH release (f) in SH-SY5Y cells. Both the cell counting kit-8 test (g) and LDH assay (h) showed that the neuroprotective effects of NGR2 was independent of cell type. The results were expressed as the mean ± SD of three independent experiments. ## indicates a significant difference from the control (). ** indicates a significant difference from the 6-OHDA treatment alone ().
