Dissection of Mechanisms of a Chinese Medicinal Formula: Danhong Injection Therapy for Myocardial Ischemia/Reperfusion Injury In Vivo and In Vitro

Figure 2

Determination of the major active ingredients in DHI involved in myocardial protection. (a) and (b) The percentage of AAR/LV and IS/AAR of different treatments. (c) and (d) Serum levels of biochemical indicators cTnI and CK-MB of each group. (e) and (m) Representative light microscopic (H&E, ×40) appearances of rat myocardial histopathological morphology for each group. AAR, area at risk; LV, left ventricle; IS, infarct size; MI/R, myocardial ischemia/reperfusion; A, hydroxysafflor yellow A; B, salvianolic acid B; C, danshensu; D, protocatechuic aldehyde; E, rosmarinic acid; DHI, Danhong injection; Com, the combination of A, B, C, D, and E. Data are presented as mean ± SD (𝑛=6). Solid circles represent individual results. ^##𝑃<0.01 versus control; *𝑃<0.05, **𝑃<0.01 versus MI/R; ^△𝑃<0.05 versus MI/R + DHI.