Figure 4: The differentially expressed 11 spots among all 3 groups on 2DE gel from a preparative gel. The 11 spots marked with red circles and ID codes expressed among all three groups. More importantly, the 11 spots in the normal and FZHY group showed the same differential expression compared with the model group. 1.0 mg of total protein was isoelectrically focused on IPG strips (pH 3–10), then separated by 12% SDS-PAGE as seconddimension. The protein spots were visualized by Coomassie Blue G250 staining.