Figure 4: CB-PIC (0 or 40  g/mL) was treated under normoxic or hypoxic conditions for various times (0, 0.5, 1, 2, 4, or 6 hrs). Western blotting was performed to determine HIF-1 , AMPK , pAMPK , pERK, ERK, and -actin expressions. (a) CB-PIC dramatically induces hypoxia-induced pAMPK and pERK expressions and decreases hypoxia inducible factor 1 (HIF-1 ) accumulation in SW620 cells under hypoxia as time-dependent manner. (b) CB-PIC induced pAMPK , and pERK expression was increased.