Research Article

Curative Effects of Thiacremonone against Acetaminophen-Induced Acute Hepatic Failure via Inhibition of Proinflammatory Cytokines Production and Infiltration of Cytotoxic Immune Cells and Kupffer Cells

Figure 3

Downregulation of proinflammatory cytokines in the liver of thiacremonone pretreated mice. (a) Mouse cytokine array panel coordinates. Nitrocellulose membranes contain 40 different anticytokine antibodies printed in duplicate. (b) Mouse cytokine array panel indicates the cytokine expression difference in the liver tissues of control mice, APAP-treated mice, and thiacremonone pretreated mice. ((c), (d), and (e)) Representative blot from three independent experiments is shown. Positive controls show the manufacturer’s internal positive control samples on the membrane. In particular, the expression levels of IL-1α, IL-7, IL-17, I-309, MIG, M-CSF, MIP-1α, and MIP-1β in thiacremonone pretreated mice were significantly decreased compared to APAP-treated mice.
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