Mulberry Extracts Alleviate Aβ_25–35-Induced Injury and Change the Gene Expression Profile in PC12 Cells

Figure 5

mRNA expression of Apaf1, Bace2, and Plcb4 genes measured by RT-PCR. Cells were pretreated with or without ME for 24h and exposed to Aβ _25–35 (20 μmol/L) for 24 h. (a) Typical mRNA bands of Apaf1, Bace2, Plcb4 and β-actin from control group, 200 μg/mL ME treatment alone group, pretreatment group with 200 μg/ml ME, and treatment group with Aβ _25–35 alone. (b) Apaf1, Bace2 and Plcb4 mRNA levels were normalized to β-actin mRNA level and presented as relative value. The values represent mean ± S.D. of the each group () of three independent experiments. (#) Significantly different from the control group (). () Significantly different from the Aβ _25–35 group ().

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