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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 157938, 10 pages
http://dx.doi.org/10.1155/2014/157938
Research Article

Neuroprotective Effect of a Formula, Moschus Combined with Borneolum Synthcticum, from Traditional Chinese Medicine on Ischemia Stroke in Rats

1The First Hospital Affiliated to Guangzhou Medical University, Guangzhou 510120, China
2The Heart Center, The First Hospital Affiliated to Lanzhou University, Lanzhou 710030, China
3School of Chinese Material Medical of Guangzhou University of Chinese Medicine, 232 East Waihuan Road, Guangzhou Higher Education Mega Center, Guangzhou, Guangdong 510006, China

Received 21 November 2013; Accepted 22 February 2014; Published 25 March 2014

Academic Editor: Pitchairaj Geraldine

Copyright © 2014 Xin-hua Xia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Moschus compatible with borneolum synthcticum is a well-known herb pair in Traditional Chinese Medicine and the present study aims to assess the neuroprotective effect of a formula composed of this herb pair on ischemia stroke in rats. The middle cerebral artery occlusion model of focal cerebral ischemia in rat was performed by using intraluminal suture method. The behavioral scores, infarct volume, and neuron ultrastructure of model and formula-treated rats were investigated after the 2 h of ischemia and 24 h of reperfusion. Meanwhile the expression levels of caspase-3, caspase-9, Bcl-2, and Bax were measured by western blot analysis. The formula treatment showed obvious neuroprotective effect according to significant decrease of the neurological scores and the infarct volumes when compared to the MCAO group. We also observed that this formula had antiapoptosis activity on neuron cell under electron microscope. Furthermore, our result supported the idea that pro- and postadministration of this formula had an antiapoptosis effect by decreasing remarkably the expression of caspase-3 and caspase-9 as well as increasing significantly the ratio of Bcl-2 to Bax . All evidences demonstrated the neuroprotective effect of this formula on ischemia stroke due to decrease of brain infract volume and modulation of the expression of apoptosis-related proteins.