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Evidence-Based Complementary and Alternative Medicine
Volume 2014, Article ID 158018, 7 pages
http://dx.doi.org/10.1155/2014/158018
Research Article

Effect of Achyranthes bidentata Blume on 3T3-L1 Adipogenesis and Rats Fed with a High-Fat Diet

1Department of East-West Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea
2Department of Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Yongin, Republic of Korea
3Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Republic of Korea
4Acupuncture & Meridian Science Research Center, Kyung Hee University, Seoul, Republic of Korea
5Division of Homeostatic Development, National Institute for Physiological Sciences, Okazaki, Japan
6Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea
7Department of Oriental Pediatrics, Kyung Hee Medical Center, Kyung Hee University, Seoul, Republic of Korea
8Department of Family Medicine, CHA University School of Medicine, Seoul, Republic of Korea
9Department of Integrative Medicine, CHA University School of Medicine, 442 Dosan-daero, Gangnam-gu, Seoul 135-948, Republic of Korea

Received 17 February 2014; Revised 14 April 2014; Accepted 22 April 2014; Published 14 May 2014

Academic Editor: Raffaele Capasso

Copyright © 2014 Sang Deog Oh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study investigated the antiobesity effect of Achyranthes bidentata Blume root water extract in a 3T3-L1 adipocyte differentiation model and rats fed with a high-fat diet. To investigate the effect of Achyranthes bidentata Blume on adipogenesis in vitro, differentiating 3T3-L1 cells in adipocyte-induction media were treated every two days with Achyranthes bidentata Blume at various concentrations (1 to 25 μg/mL) for eight days. We found that Achyranthes bidentata Blume root inhibited 3T3-L1 adipocyte differentiation without affecting cell viability, and Western blot analysis revealed that phospho-Akt expression was markedly decreased, whereas there was no significant change in perilipin expression. Furthermore, administration of Achyranthes bidentata Blume root (0.5 g/kg body weight for six weeks) to rats fed with a high-fat diet significantly reduced body weight gain without affecting food intake, and the level of triglyceride was significantly decreased when compared to those in rats fed with only a high-fat diet. These results suggest that Achyranthes bidentata Blume root water extract could have a beneficial effect on inhibition of adipogenesis and controlling body weight in rats fed with a high-fat diet.