Effect of Nourishing “Yin”-Removing “Fire” Chinese Herbal Mixture on Hypothalamic NKB/NK3R Expression in Female Precocious Rats

Figure 5

Effects of Chinese herb mixture on hypothalamic NKB/NK3R mRNA expression by real-time PCR. (a) The expression of NKB mRNA in M has significantly increased compared to N (𝑃<0.01) and decreased in CHM compared to M (𝑃<0.01). (b) The picture shows that the expression of NK3R mRNA in M was higher than in N (𝑃<0.01), and that Chinese herb mixture was downregulated NK3R mRNA expression compared to M (𝑃<0.05). Results analysis used the ratio of NKB or NK3R/𝛽-actin (𝑛=5 per group) expressed as the mean with SEM. N: normal, M: model, MS: saline, and CHM: Chinese herb mixture. ^∗∗𝑃<0.01 versus N; ^#𝑃<0.05 versus M; ^##𝑃<0.01 versus M.

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