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Evidence-Based Complementary and Alternative Medicine
Volume 2014 (2014), Article ID 231258, 8 pages
http://dx.doi.org/10.1155/2014/231258
Research Article

Traditional Japanese Medicine Daikenchuto Improves Functional Constipation in Poststroke Patients

1Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-Machi, Aoba Ward, Sendai City, Miyagi 980-8575, Japan
2Department of Kampo Medicine, Tohoku University Hospital, 1-1 Seiryo-Machi, Aoba Ward, Sendai City, Miyagi 980-8574, Japan
3Comprehensive Education Center for Community Medicine, Tohoku University Graduate School of Medicine, 2-1 Seiryo-Machi, Aoba Ward, Sendai City, Miyagi 980-8575, Japan
4National Yonezawa Hospital, 26100-1 Oh-Aza Misawa, Yonezawa City, Yamagata 992-1202, Japan
5Ishinomaki Rehabilitation Hospital, 1-2-21 Kadonowaki-cho, Ishinomaki City, Miyagi 986-0834, Japan
6Miyagi Rifu Ekisaikai Hospital, 51 Morigo Aza Shintaishido, Rifu Town, Miyagi 981-0103, Japan
7Hikarigaoka Spellman Hospital, 6-7-1 Higashi-Sendai, Miyagino Ward, Sendai City, Miyagi 983-0833, Japan
8National Hachinohe Hospital, 3-13-1 Fukiage, Hachinohe City, Aomori 031-0003, Japan
9Wakuya Medical and Welfare Center, 278 Wakuya Aza Nakakonan, Wakuya Town, Miyagi 987-0121, Japan
10Department of Education and Support for Community Medicine, Tohoku University Hospital, 1-1 Seiryo-Machi, Aoba Ward, Sendai City, Miyagi 980-8574, Japan
11Center for Traditional Asian Medicine and Home Healthcare, Southern Tohoku General Hospital, 1-2-5 Satonomori, Iwanuma City, Miyagi 989-2483, Japan

Received 25 March 2014; Revised 5 May 2014; Accepted 13 May 2014; Published 25 June 2014

Academic Editor: Toku Takahashi

Copyright © 2014 Takehiro Numata et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Poststroke patients with functional constipation, assessed by the Rome III criteria, from 6 hospitals were recruited in a study on the effects of the traditional Japanese medicine Daikenchuto (DKT) on constipation. Thirty-four patients (17 men and 17 women; mean age: 78.1 ± 11.6 years) were randomly assigned to 2 groups; all patients received conventional therapy for constipation, and patients in the DKT group received 15 g/day of DKT for 4 weeks. Constipation scoring system (CSS) points and the gas volume score (GVS) (the measure of the intestinal gas volume calculated from plain abdominal radiographs) were recorded before and after a 4-week observation period. The total score on the CSS improved significantly in the DKT group compared to the control . In addition, scores for some CSS subcategories (frequency of bowel movements, feeling of incomplete evacuation, and need for enema/disimpaction) significantly improved in the DKT group (, , and , resp.). The GVS was also significantly reduced in the DKT group compared to the control . DKT in addition to conventional therapy is effective in treating functional constipation in poststroke patients. This study was a randomized controlled trial and was registered in the UMIN Clinical Trial Registry (no. UMIN000007393).

1. Introduction

There were over 1.34 million cerebrovascular patients in 2008 reported by the Japanese Ministry of Health, Labour, and Welfare [1]. Constipation is one of the complications seen in poststroke patients. Stratified by stroke severity on the National Institutes of Health Stroke Scale, the reported incidence of constipation in poststroke patients is 38.9% to 88.2% [2]. Functional constipation is thought to originate from decreased gastrointestinal motility as well as from decreased autonomic nervous system efficiency, impaired physical activity, abdominal muscle weakness secondary to hemiplegia, and diet [3]. Conventional therapy to control constipation involves the use of laxatives or stimulant purgatives, and these drugs are often used in the long term in chronic constipation patients [4]. However, patients can develop a tolerance to laxatives or stimulant purgatives, and paralytic ileus occasionally occurs in the clinical setting, even with conventional therapy [5].

DKT has historically been used to treat gastrointestinal dysfunction with abdominal coldness and pain in many East Asian countries, including Japan and China [6]. Recently, it has also been used to prevent ileus after gastrointestinal surgery and to treat irritable bowel syndrome [7]. Horiuchi et al. reported that DKT significantly improved abdominal bloating and pain and reduced intestinal gas volume in patients with intractable functional constipation [8]. Physiological reactions to the administration of DKT have been reported as promoting gastrointestinal motility [913] and increasing intestinal blood perfusion [1419]. DKT’s effectiveness in treating defecation disorders in patients with cerebrovascular disease is commonly observed in the clinical setting. Potential mechanisms underlying the physiological responses to DKT have been investigated in animal models and include elevated levels of plasma vasoactive intestinal polypeptide [14, 17, 20], substance P [14, 17, 21, 22], motilin [2325], and acetylcholine [10, 11, 13, 2628], which promote gastrointestinal motility, as well as calcitonin gene-related peptide (CGRP) [14, 15, 17, 21] and adrenomedullin [15, 16, 29, 30], which increase intestinal blood flow. Poststroke patients are at risk for arteriosclerosis and often experience abdominal pain accompanied by a cold sensation in the abdomen associated with low blood perfusion in the mesenteric arteries. DKT has been used to treat defecation disorders with abdominal coldness and pain caused by decreased intestinal motility and blood flow. We previously reported that administration of DKT increased blood flow in the superior mesenteric artery and promoted intestinal peristalsis in healthy subjects [18, 19]. Sato et al. reported that DKT significantly increased plasma CGRP levels in healthy subjects [21]. Therefore, plasma CGRP may be a useful biomarker to evaluate the effects of DKT on intestinal blood flow.

This study aimed to investigate the efficacy of DKT in treating functional constipation in poststroke patients. In addition, this study investigated the impact of DKT therapy on CGRP concentration.

2. Methods

2.1. Subject Eligibility Criteria

Eligible patients were aged 20 to 99 years of both genders, had been diagnosed with functional constipation according to the Rome III criteria [31], and remained stable over a 6-month period from the onset of cerebral hemorrhage, cerebral infarction, and subarachnoid hemorrhage. Patients received nutrition orally or through a nasogastric or gastrostomy tube. Patients with concurrent diabetes were required to have an HbA1c (NGSP) less than 9%.

2.2. Subject Exclusion Criteria

Patients meeting or diagnosed with any of the following criteria were excluded: risk of intestinal adhesion following abdominal surgery, inflammatory bowel disease, or malignant gastrointestinal disease; hypoxic encephalopathy or myelopathy; history of interstitial pneumonia; liver and/or kidney dysfunction; cancer; and neurodegenerative disease, such as Parkinson’s disease or spinocerebellar degeneration. However, patients who underwent laparoscopic cholecystectomy or underwent percutaneous endoscopic gastrostomy were not excluded because the invasiveness of the operation was minimal.

2.3. Patient Recruitment

From September 2012 to December 2013, eligible subjects were recruited from 6 hospitals: National Yonezawa Hospital, Ishinomaki Rehabilitation Hospital, National Hachinohe Hospital, Hikarigaoka Spellman Hospital, Miyagi Rifu Ekisaikai Hospital, and Wakuya Medical and Welfare Center.

2.4. Logistics

Subjects were randomly assigned to the DKT group or the control group. The study protocol was conducted in accordance with the Declaration of Helsinki and was approved by the Institutional Review Boards of Tohoku University Hospital and the 6 collaborating hospitals. Written informed consent was obtained from all patients or their families.

2.5. Trial Methods

The study protocol included an intention to treat analysis. The control group underwent conventional therapy for constipation, such as laxative administration, enemas, and disimpaction. In addition to conventional therapy, the DKT group continuously received 5.0 g of Daikenchuto extract granules (TJ-100, Tsumura & Co., Tokyo, Japan) 3 times a day before meals for 4 weeks. Each clinical parameter was measured before and after the 4-week trial. Fifteen grams of TJ-100 (DKT) extract granules contains a dried herbal extract mixture in the following proportions: Ginseng radix (Araliaceae, Panax ginseng C.A. Meyer, Radix) (3.0 g), processed ginger root (Zingiberaceae, Zingiber officinale Roscoe, rhizoma) (5.0 g), Zanthoxylum fruit (Rutaceae, Zanthoxylum piperitum De Candolle, pericarpium) (2.0 g), and saccharum granorum (the candy produced from maltose) (10.0 g). This formulation is registered in the Japanese Pharmacopoeia Sixteenth Edition [32]. The production and supply processes for TJ-100 comply with good manufacturing practice standards for Kampo products and have been approved by the Japanese Ministry of Health, Labour, and Welfare.

2.6. Evaluation of Clinical Symptoms
2.6.1. Activities of Daily Living

The Barthel Index was recorded for each patient at study enrollment to assess activities of daily living [33].

2.6.2. Clinical Constipation Scores

Clinical scores for constipation were recorded before and after the 4-week trial period using the constipation scoring system (CSS, see the appendix) [34]. Questionnaires concerning constipation were administered to patients; however, if the patients could not completely answer the question, their families or nurses evaluated the questions depending on the objective findings (i.e., painful evacuation effort or abdominal pain before defecation was evaluated by family members or nurses using the patients’ facial expressions; feeling of incomplete evacuation was evaluated with abdominal fullness after defecation). Because it was difficult to evaluate Q5 (“Time: minutes in lavatory per attempt”) in the CSS for bedridden subjects using diapers, we removed Q5 from the statistical analysis. Evaluations before and after the administration of DKT were performed by the same family member or nurse with blinding of DKT administration.

2.6.3. Plain Abdominal Radiography

Plain abdominal radiographs of fasting patients in a supine position were obtained before and after the trial period. The gas volume score (GVS) was calculated by Koide’s method [35] using ImageJ [36] (Figure 1).

fig1
Figure 1: Estimation of gas volume score (GVS). Plain abdominal radiographs obtained from fasting subjects were converted to digital data. The data were read using ImageJ, an image analysis program, and intestinal gas was traced using the program. (a) Tracing image and pixel count of the gas was 3,533 in this patient. (b) The window of abdominal area. The rectangular area was measured as the area between the inferior right side margin of the diaphragm, the inner costal margin, and the superior border of the pubic symphysis. The pixel count of the rectangular area was calculated as 92,968 in (b). GVS was calculated as (a)/(b)%; therefore, the GVS of this image is “.”
2.6.4. Blood Sampling

General blood counts and biochemistry tests were performed in fasting patients before and after the trial period to assess potential adverse effects. Blood sample portions were stored in EDTA-2Na tubes. Samples were centrifuged (3000 rev/10 min), and 0.5 mL of plasma was collected and stored at −20°C. The concentration of plasma CGRP was quantified using the Human CGRP Elisa Kit (MyBioSource, Inc., San Diego, USA) tested by SRL, Inc., Tokyo, Japan.

2.6.5. Statistical Analysis

Statistical analysis was performed using SPSS software (ver. 16, SPSS Japan Inc., Tokyo, Japan). Baseline comparisons of group differences were conducted using the independent samples t-test for continuous variables and the chi-square test for categorical variables. Measurement of the mean and standard deviation (SD) was performed at baseline and at the endpoint for all parameters. Comparisons between the DKT and control groups were performed by two-way analysis of variance (ANOVA). Changes within groups before and after the trial period were compared using the paired t-test when the intergroup difference was significant. Correlation between age and the CSS points was analyzed by coefficient of product-moment correlation (Pearson correlation coefficient). P values <0.05 were considered significant.

3. Results

From September 2012 to December 2013, 34 subjects (17 men and 17 women; mean age: 78.1 ± 11.6 years) at 6 hospitals participated in the study. Patients were randomly assigned to 2 groups (control group or DKT group). The demographic characteristics, CSS, and GVS of each group at baseline are shown in Table 1. There was no significant difference between groups in characteristics, the way of nutrition intake, CSS, or GVS at baseline.

tab1
Table 1: Baseline population demographics of DKT and control groups.

3.1. Changes in Clinical Constipation Scores

All 34 subjects completed the CCS questionnaire before and after the observation period, and results are summarized in Table 2. There was no significant correlation between age and the CSS points on the baseline () (, ). Significant differences in the CSS scores were observed between the 2 groups (two-way ANOVA, ). In the DKT group, the CSS scores significantly improved from 8.0 ± 3.1 to 6.0 ± 3.1 points (paired t-test, ). There was no significant correlation between age and the changes of the CSS scores for subjects in DKT group () (, ). The control group did not show any significant improvement (Table 2). CSS subcategory findings are summarized for both groups in Table 3. Among the CSS subcategories, there were significant differences between the DKT and control groups using two-way ANOVA for the following questions: Q1 (frequency of bowel movements; ), Q3 (feeling of incomplete evacuation; ), and Q6 (need for drugs/enema/disimpaction; ). In the DKT group, the constipation scores significantly decreased over the trial period for Q1 (), Q3 (), and Q6 (). The control group, however, did not show any significant changes (Table 3). Overall, the average change of 1 point in the score for Q1 means an improvement in defecation frequency from “once per week” to “2 times per week” or “less than once per week” to “once per week” in the clinical setting. The average change of 0.4 points in the scores for Q3 and Q6 means that digital assistance or enemas were no longer necessary for approximately 30% of the patients in the DKT group.

tab2
Table 2: Clinical constipation scores in both groups at baseline and endpoint.
tab3
Table 3: Constipation scoring system (CSS) subcategory scores in both groups at baseline and endpoint.

3.2. Changes in Gas Volume Score

Figure 2 summarizes changes in the GVS before and after the observation period for both groups. There was a significant difference between the 2 groups (two-way ANOVA; ), and the intragroup comparison revealed a significant decrease in the DKT group from 16.3 ± 6.7% to 9.9 ± 6.0% () while the control group did not show any significant changes (). Representative abdominal radiographs of a patient before and after DKT administration show reduced intestinal gas volume (Figures 3(a) and 3(b)). In this case, DKT administration reduced the GVS from 26.0% to 12.3%.

231258.fig.002
Figure 2: Changes in the gas volume score (GVS). Two-way ANOVA showed a significant difference between the groups (). In the DKT group, the GVS significantly improved from 16.3 ± 6.7% to 9.9 ± 6.0% (paired t-test; ), and in the control group it changed from 14.4 ± 7.1% to 13.5 ± 8.0% with no significance (paired t-test; ).
fig3
Figure 3: (a) Plain abdominal radiograph of an 86-year-old man prior to Daikenchuto administration. The gas volume score (GVS) was calculated as 26.0%. (b) Plain abdominal radiograph of an 86-year-old man after 4 weeks of Daikenchuto administration. The gas volume score (GVS) was calculated as 12.3%.

3.3. Changes in Plasma Calcitonin Gene-Related Peptide Concentrations

In the DKT group, the initial and final CGRP concentrations were 409 ± 482 pg/mL and 452 ± 574 pg/mL, respectively. In the control group, the initial and final values were 270 ± 172 pg/mL and 251 ± 118 pg/mL, respectively. There was no significant difference between the 2 groups in plasma CGRP (two-way ANOVA; ).

3.4. Adverse Effects

Notable adverse effects, such as itching, gastrointestinal symptoms, other subjective symptoms, and abnormalities in blood counts and blood biochemistry, were not observed during and after DKT administration.

4. Discussion

This study shows that DKT in addition to conventional therapy for functional constipation significantly improved the CSS scores and significantly reduced the GVS in poststroke patients. The incidence of adverse effects associated with DKT extract, such as gastrointestinal discomfort and liver dysfunction, has been reported as 1.9% in prior studies [37], but no adverse effects were observed during the 4-week treatment period in the present study. Functional constipation has a complex pathophysiology, and intestinal function is controlled by the autonomic nervous system; consequently, therapeutic protocols are limited in poststroke patients [38, 39]. Several clinical studies of DKT therapy for constipation have been reported, but almost all of these were limited to healthy subjects or were case series. The present study was a prospective randomized controlled trial for functional constipation in patients with stroke-related morbidity and therefore could show stronger evidence than previous reports of the clinical effects of DKT.

In a prior clinical study, it was reported that DKT extract improved colorectal function in patients diagnosed with Parkinson’s disease [40]. Another study reported that administration of DKT to patients with chronic intractable constipation improved abdominal bloating and pain symptoms [8]. The present study similarly found improvement in clinical constipation scores and GVS. Numerous studies have investigated the active ingredients and mechanisms underlying the improved intestinal motility. Intestinal contraction may be induced by DKT through the cholinergic nervous system via serotonin receptors [13, 27, 28], motilin activity [23, 24], and the transient receptor potential vanilloid type 1 channel [11, 41]. Satoh et al. reported that Zanthoxylum fruit and maltose, ingredients in DKT, improved delayed propulsion in the small intestine. Zanthoxylum fruit also improved delayed propulsion in the distal colon. Endogenous cholecystokinin secretion resulting from maltose administration may play a role in the effect of DKT [42]. These reports describe the possible mechanisms through which DKT promotes intestinal movement and explain some aspects of the improvement in the CSS scores and the reduction of GVS noted in our study.

Some studies reported that DKT extract increased CGRP in healthy subjects [21, 25]. In another report, DKT did not change CGRP levels in patients with constipation secondary to palliative morphine therapy for cancer [24]. In the present study, changes in CGRP did not reach statistical significance. Several mechanisms may explain this lack of change in CGRP levels in the DKT group. Plasma CGRP is notably unstable [43]. An elevation following DKT administration may have been obscured by factors such as testing procedures, individual differences, daily fluctuations, and day-to-day variations. Furthermore, although some studies confirmed elevated CGRP immediately after DKT administration [21, 25], the CGRP level may be too unstable to be used as a target factor for evaluating the effects of DKT. DKT is thought to affect the promotion of intestinal motility and intestinal blood flow. Increase in intestinal blood flow is believed to be mediated through adrenomedullin and CGRP or through the transient receptor potential ankyrin 1 channel [16, 29, 30]. The mechanisms promoting intestinal motility and blood flow have complex interactions, which may be altered further by disease pathology, environment, and individual differences. The present results of improved constipation following DKT administration are overall consistent with the findings of prior studies, despite the lack of significant change in CGRP levels.

4.1. Limitations

The small sample size is the first limitation of the present study. The CGRP level tended to differ between the groups (ANOVA, ); a larger sample size could determine the significance of this difference. In addition, participants were limited to hospitalized patients; therefore, patients who were hemiplegic, yet stable enough to receive outpatient care, were not included. As a result, the population was skewed toward patients with low activities of daily living. Third, there are no objective parameters for abdominal coldness at present. Ultrasound assessment of blood flow in the superior mesenteric artery was nearly impossible in poststroke patients with constipation owing to the presence of intestinal gas. Finally, the placebo effect of oral administration cannot be overlooked. A randomized double-blind comparative study using a placebo would be ideal and would eliminate the placebo effect. DKT includes 4 crude herbs and has a sweet and hot flavor. It will be difficult to produce a placebo without bioactivity that has a smell and flavor similar to DKT. Accordingly, the present study did not use a placebo control but rather compared the effects of DKT administration plus conventional treatment to conventional treatment alone.

5. Conclusions

Administration of DKT extract in conjunction with conventional therapy to treat functional constipation in poststroke patients improved clinical constipation scores and reduced intestinal gas volume. Results of this study show that DKT is effective for defecation control in poststroke patients.

Appendix

Constipation Scoring System (CSS) [34]

Minimum score, 0; Maximum score, 30, the numbering starting from zero represents the scores.(1) Frequency of bowel movements(0) 1-2 times per 1-2 days(1) 2 times per week(2) Once per week(3) Less than once per week(4) Less than once per month(2) Difficulty: painful evacuation effort(3) Completeness: feeling incomplete evacuation(4) Pain: abdominal pain(0) Never(1) Rarely(2) Sometimes(3) Usually(4) Always(5) Time: minutes in lavatory per attempt(0) Less than 5(1) 5−10(2) 10−20(3) 20−30(4) More than 30(6) Assistance: type of assistance(0) Without assistance(1) Stimulative laxatives(2) Digital assistance or enema(7) Failure: unsuccessful attempts for evacuation per 24 hours(0) Never(1) 1–3(2) 3–6(3) 6–9(4) More than 9(8) History: duration of constipation (yr)(0) 0(1) 1–5(2) 5–10(3) 10–20(4) More than 20

Conflict of Interests

All authors declare no personal competing financial or nonfinancial interests in this study; however, Tohoku University Graduate School of Medicine received a grant from Tsumura Co. Ltd., the manufacturer of TJ-100.

Authors’ Contribution

Takehiro Numata took part in planning the study, performed the data analysis, and wrote the paper. Shin Takayama and Koh Iwasaki were the original proposers of the study and were involved in developing the protocol and paper preparation. Muneshige Tobita, Shuichi Ishida, Dai Katayose, Mitsutoshi Shinkawa, Takashi Oikawa, and Takanori Aonuma took part in recruiting subjects and laboratory management in their hospitals. Soichiro Kaneko, Junichi Tanaka, and Seiki Kanemura helped to plan the study and provided advice related to writing the paper. Tadashi Ishii and Nobuo Yaegashi were responsible for the study design and execution and assisted in writing the paper. All authors read and approved the final paper.

Acknowledgments

The present study was conducted using a Grant-in-Aid for Scientific Research (Academic Research Grant, no. 23590867). The authors sincerely appreciate the individuals who participated in the trial throughout the entire study, as well as their families. They are grateful to the staff at National Yonezawa Hospital, Ishinomaki Rehabilitation Hospital, Miyagi Rifu Ekisaikai Hospital, Hikarigaoka Spellman Hospital, National Hachinohe Hospital, and Wakuya Medical and Welfare Center for their help with data collection.

References

  1. Ministry of Health, Labour and Welfare, “Summary of Patient Survey, 2008. 5 Estimated Number of Patients Receiving Medical Treatment for Selected Diseases,” http://www.mhlw.go.jp/english/database/db-hss/dl/sps_2008_05.pdf.
  2. Y. Su, X. Zhang, J. Zeng et al., “New-onset constipation at acute stage after first stroke: incidence, risk factors, and impact on the stroke outcome,” Stroke, vol. 40, no. 4, pp. 1304–1309, 2009. View at Publisher · View at Google Scholar · View at Scopus
  3. G. Basilisco and M. Coletta, “Chronic constipation: a critical review,” Digestive and Liver Disease, vol. 45, no. 11, pp. 886–893, 2013. View at Publisher · View at Google Scholar · View at Scopus
  4. M. Coggrave, C. Norton, and J. D. Cody, “Management of faecal incontinence and constipation in adults with central neurological diseases,” Cochrane Database of Systematic Reviews, vol. 2014, no. 1, Article ID CD002115, 2014. View at Publisher · View at Google Scholar · View at Scopus
  5. K. Krogh, C. Mosdal, H. Gregersen, and S. Laurberg, “Rectal wall properties in patients with acute and chronic spinal cord lesions,” Diseases of the Colon and Rectum, vol. 45, no. 5, pp. 641–649, 2002. View at Publisher · View at Google Scholar · View at Scopus
  6. Z. Zhang, Synopsis of Prescriptions of the Golden Chamber, New World Press, Beijing, China, 1987.
  7. H. Kawahara and K. Yanaga, “The herbal medicine Dai-Kenchu-To directly stimulates colonic motility,” Surgery Today, vol. 39, no. 2, pp. 175–177, 2009. View at Publisher · View at Google Scholar · View at Scopus
  8. A. Horiuchi, Y. Nakayama, and N. Tanaka, “Effect of traditional Japanese medicine, Daikenchuto (TJ-100) in patients with chronic constipation,” Gastroenterology Research, vol. 3, no. 4, pp. 151–155, 2010. View at Publisher · View at Google Scholar
  9. Y. Furukawa, Y. Shiga, N. Hanyu et al., “Effect of Chinese herbal medicine on gastrointestinal motility and bowel obstruction,” The Japanese Journal of Gastroenterological Surgery, vol. 28, no. 4, pp. 956–960, 1995 (Japanese). View at Publisher · View at Google Scholar
  10. X. L. Jin, C. Shibata, H. Naito et al., “Intraduodenal and intrajejunal administration of the herbal medicine, Dai-kenchu-tou, stimulates small intestinal motility via cholinergic receptors in conscious dogs,” Digestive Diseases and Sciences, vol. 46, no. 6, pp. 1171–1176, 2001. View at Publisher · View at Google Scholar · View at Scopus
  11. D. Kikuchi, C. Shibata, H. Imoto, T. Naitoh, K. Miura, and M. Unno, “Intragastric Dai-Kenchu-To, a Japanese herbal medicine, stimulates colonic motility via transient receptor potential cation channel subfamily V member 1 in dogs,” The Tohoku Journal of Experimental Medicine, vol. 230, no. 4, pp. 197–204, 2013. View at Publisher · View at Google Scholar · View at Scopus
  12. N. Manabe, M. Camilleri, A. Rao et al., “Effect of Daikenchuto (TU-100) on gastrointestinal and colonic transit in humans,” American Journal of Physiology: Gastrointestinal and Liver Physiology, vol. 298, no. 6, pp. G970–G975, 2010. View at Publisher · View at Google Scholar · View at Scopus
  13. C. Shibata, I. Sasaki, H. Naito, T. Ueno, and S. Matsuno, “The herbal medicine Dai-Kenchu-Tou stimulates upper gut motility through cholinergic and 5-hydroxytryptamine 3 receptors in conscious dogs,” Surgery, vol. 126, no. 5, pp. 918–924, 1999. View at Publisher · View at Google Scholar · View at Scopus
  14. T. Kono, T. Koseki, S. Chiba et al., “Colonic vascular conductance increased by Daikenchuto via calcitonin gene-related peptide and receptor-activity modifying protein 1,” Journal of Surgical Research, vol. 150, no. 1, pp. 78–84, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. T. Kono, Y. Omiya, Y. Hira et al., “Daikenchuto (TU-100) ameliorates colon microvascular dysfunction via endogenous adrenomedullin in Crohn's disease rat model,” Journal of Gastroenterology, vol. 46, no. 10, pp. 1187–1196, 2011. View at Google Scholar · View at Scopus
  16. T. Kono, A. Kaneko, Y. Omiya, K. Ohbuchi, N. Ohno, and M. Yamamoto, “Epithelial transient receptor potential ankyrin 1 (TRPA1)-dependent adrenomedullin upregulates blood flow in rat small intestine,” American Journal of Physiology: Gastrointestinal and Liver Physiology, vol. 304, no. 4, pp. G428–G436, 2013. View at Publisher · View at Google Scholar · View at Scopus
  17. P. Murata, Y. Kase, A. Ishige, H. Sasaki, S. Kurosawa, and T. Nakamura, “The herbal medicine Dai-kenchu-to and one of its active components [6]-shogaol increase intestinal blood flow in rats,” Life Sciences, vol. 70, no. 17, pp. 2061–2070, 2002. View at Publisher · View at Google Scholar · View at Scopus
  18. S. Takayama, T. Seki, M. Watanabe et al., “The herbal medicine Daikenchuto increases blood flow in the superior mesenteric artery,” The Tohoku Journal of Experimental Medicine, vol. 219, no. 4, pp. 319–330, 2009. View at Publisher · View at Google Scholar · View at Scopus
  19. S. Takayama, T. Seki, M. Watanabe et al., “The effect of warming of the abdomen and of herbal medicine on superior mesenteric artery blood flow—a pilot study,” Forschende Komplementarmedizin, vol. 17, no. 4, pp. 195–201, 2010. View at Publisher · View at Google Scholar · View at Scopus
  20. T. Nagano, H. Itoh, and M. Takeyama, “Effects of Dai-kenchu-to on levels of 5-hydroxytryptamine (serotonin) and vasoactive intestinal peptides in human plasma,” Biological and Pharmaceutical Bulletin, vol. 23, no. 3, pp. 352–353, 2000. View at Google Scholar · View at Scopus
  21. Y. Sato, F. Katagiri, S. Inoue, H. Itoh, and M. Takeyama, “Dai-kenchu-to raises levels of calcitonin gene-related peptide and substance P in human plasma,” Biological and Pharmaceutical Bulletin, vol. 27, no. 11, pp. 1875–1877, 2004. View at Publisher · View at Google Scholar · View at Scopus
  22. Y. Suzuki, H. Itoh, R. Yamamura, R. Tatsuta, Y. Sato, and M. Takeyama, “Significant increase in salivary substance P level after a single oral dose of Japanese herbal medicine Dai-kenchu-to in humans,” Biomedicine & Aging Pathology, vol. 2, no. 3, pp. 81–84, 2012. View at Publisher · View at Google Scholar · View at Scopus
  23. T. Nagano, H. Itoh, and M. Takeyama, “Effect of Dai-kenchu-to on levels of 3 brain-gut peptides (motilin, gastrin and somatostatin) in human plasma,” Biological and Pharmaceutical Bulletin, vol. 22, no. 10, pp. 1131–1133, 1999. View at Google Scholar · View at Scopus
  24. Y. Satoh, H. Itoh, and M. Takeyama, “Daikenchuto raises plasma levels of motilin in cancer patients with morphine-Induced constipation,” Journal of Traditional Medicines, vol. 27, no. 3, pp. 115–121, 2010. View at Google Scholar
  25. Y. Sato, S. Inoue, F. Katagiri, H. Itoh, and M. Takeyama, “Effects of pirenzepine on Dai-kenchu-to-induced elevation of the plasma neuropeptide levels in humans,” Biological and Pharmaceutical Bulletin, vol. 29, no. 1, pp. 166–171, 2006. View at Publisher · View at Google Scholar · View at Scopus
  26. H. Fukuda, C. Chen, C. Mantyh, K. Ludwig, T. N. Pappas, and T. Takahashi, “The herbal medicine, Dai-Kenchu-To, accelerates delayed gastrointestinal transit after the operation in rats,” Journal of Surgical Research, vol. 131, no. 2, pp. 290–295, 2006. View at Publisher · View at Google Scholar · View at Scopus
  27. K. Satoh, K. Hashimoto, T. Hayakawa et al., “Mechanism of atropine-resistant contraction induced by Dai-kenchu-to in guinea pig ileum,” The Japanese Journal of Pharmacology, vol. 86, no. 1, pp. 32–37, 2001. View at Publisher · View at Google Scholar · View at Scopus
  28. K. Satoh, T. Hayakawa, Y. Kase et al., “Mechanisms for contractile effect of Dai-kenchu-to in isolated guinea pig ileum,” Digestive Diseases and Sciences, vol. 46, no. 2, pp. 250–256, 2001. View at Publisher · View at Google Scholar · View at Scopus
  29. A. Kaneko, T. Kono, N. Miura, N. Tsuchiya, and M. Yamamoto, “Preventive effect of TU-100 on a type-2 model of colitis in mice: possible involvement of enhancing adrenomedullin in intestinal epithelial cells,” Gastroenterology Research and Practice, vol. 2013, Article ID 384057, 8 pages, 2013. View at Publisher · View at Google Scholar
  30. T. Kono, A. Kaneko, Y. Hira et al., “Anti-colitis and -adhesion effects of Daikenchuto via endogenous adrenomedullin enhancement in Crohn's disease mouse model,” Journal of Crohn's and Colitis, vol. 4, no. 2, pp. 161–170, 2010. View at Publisher · View at Google Scholar · View at Scopus
  31. D. A. Drossman and E. Corazziari, Rome III : The Functional Gastrointestinal Disorders, Degnon Associates, Virginia, Va, USA, 3rd edition, 2006.
  32. The Japanese Pharmacopoeia, the Electronic Version, 16th edition, 2011, http://jpdb.nihs.go.jp/jp16e/.
  33. F. I. Mahoney and D. W. Barthel, “Functional evaluation: the barthel index,” Maryland State Medical Journal, vol. 14, pp. 61–65, 1965. View at Google Scholar · View at Scopus
  34. F. Agachan, T. Chen, J. Pfeifer, P. Reissman, and S. D. Wexner, “A constipation scoring system to simplify evaluation and management of constipated patients,” Diseases of the Colon and Rectum, vol. 39, no. 6, pp. 681–685, 1996. View at Publisher · View at Google Scholar · View at Scopus
  35. A. Koide, T. Yamaguchi, T. Odaka et al., “Quantitative analysis of bowel gas using plain abdominal radiograph in patients with irritable bowel syndrome,” The American Journal of Gastroenterology, vol. 95, no. 7, pp. 1735–1741, 2000. View at Publisher · View at Google Scholar · View at Scopus
  36. “ImageJ Image Processing and Analysis in Java,” http://imagej.nih.gov/ij/.
  37. Y. Katori, M. Tsukamoto, and H. Agenosono, “Investigation of the frequency of adverse drug reaction to Tsumura Daikenchuto extract granules for ethical use in Japan,” Progress in Medicine, vol. 32, no. 9, pp. 1973–1982, 2012 (Japanese). View at Google Scholar
  38. K. Winge, D. Rasmussen, and L. M. Werdelin, “Constipation in neurological diseases,” Journal of Neurology Neurosurgery and Psychiatry, vol. 74, no. 1, pp. 13–19, 2003. View at Publisher · View at Google Scholar · View at Scopus
  39. S. F. Lim and C. Childs, “A systematic review of the effectiveness of bowel management strategies for constipation in adults with stroke,” International Journal of Nursing Studies, vol. 50, no. 7, pp. 1004–1010, 2013. View at Publisher · View at Google Scholar · View at Scopus
  40. R. Sakakibara, T. Odaka, Z. Lui et al., “Dietary herb extract Dai-kenchu-to ameliorates constipation in parkinsonian patients (Parkinson's disease and multiple system atrophy),” Movement Disorders, vol. 20, no. 2, pp. 261–262, 2005. View at Publisher · View at Google Scholar · View at Scopus
  41. Y. Tokita, M. Yamamoto, K. Satoh et al., “Possible involvement of the transient receptor potential vanilloid type 1 channel in postoperative adhesive obstruction and its prevention by a kampo (traditional Japanese) medicine, Daikenchuto,” Journal of Pharmacological Sciences, vol. 115, no. 1, pp. 75–83, 2011. View at Publisher · View at Google Scholar · View at Scopus
  42. K. Satoh, Y. Kase, M. Yuzurihara, K. Mizoguchi, K. Kurauchi, and A. Ishige, “Effect of Dai-kenchu-to (Da-Jian-Zhong-Tang) on the delayed intestinal propulsion induced by chlorpromazine in mice,” Journal of Ethnopharmacology, vol. 86, no. 1, pp. 37–44, 2003. View at Publisher · View at Google Scholar · View at Scopus
  43. H. Takami, J.-I. Shikata, H. Horie, J. Horiuchi, H. Sakurai, and K. Ito, “Radioimmunoassay of plasma calcitonin gene-related peptide (CGRP) levels in patients with endocrine tumor,” Japanese Journal of Cancer and Chemotherapy, vol. 16, no. 6, pp. 2219–2225, 1989 (Japanese). View at Google Scholar · View at Scopus