The Spectroscopy Study of the Binding of an Active Ingredient of Dioscorea Species with Bovine Serum Albumin with or without Co²⁺ or Zn²⁺

Figure 2

The fluorescence spectra of BSA by DIO in the absence and presence of Co²⁺ or Zn²⁺. (a) BSA-DIO system; (b) BSA-DIO-Co²⁺ system; (c) BSA-DIO-Zn²⁺ system. From a to j, the concentration of DIO was varied from 0 to 9 × 10⁻⁶ mol·L⁻¹ at increments of 1 × 10⁻⁶ mol·L⁻¹. k: DIO only, 9 × 10⁻⁶ mol·L⁻¹. [BSA] = [Co²⁺] = [Zn²⁺] = 5 × 10⁻⁷ mol·L⁻¹, 𝑇=298 K.

(a)

(b)

(c)